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Welcome to The Cell Culture and Tissue Core Lab

The Cell Culture and Tissue Core Lab provides immortalized lymphoblast and fibroblast lines, mononuclear cell isolation and processing, as well as new tissue and cell culture services.

The Specimen Collection, Processing and Point of Care Core Lab provides the following services:

  • DNA Isolation
    - Whole blood
    - Frozen whole blood
    - Cultured cells
    - Spit kits (saliva swabs for infants)
    - Buccal swabs
    - Mouthwash
    - Blood collection cards
    - Solid tissue (umbilical cords, paraffin blocks)
  • EBV Lymphocyte Transformation - Lymphocyte transformation, culture, and cryopreservation
    - Lymphocyte collection and freezing
    - Transformation of cryopreserved lymphocytes
  • Fibroblast culture and cryopreservation
  • Specialized Cell Isolation
    - Peripheral Blood Mononuclear Cells (PBMCs)
    - Urinary Leukocytes
    - Primary cell culture from tissue

New tissue and cell culture services are in development (e.g., human adipose and adipocyte, as part of collaboration with Institute for Diabetes, Obesity, and Metabolism).

Contacts

For more information, please contact the respective Technical Directors.

Shiv Kapoor, Ph.D., Penn Technical Director
Phone: (215) 662-3928
Email: shiv@gcrc.upenn.edu

David Stokes, Ph.D., CHOP Technical Director
Phone: (215) 590-4752
Email: stokesdg@email.chop.edu

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