University of Pennsylvania | School of Medicine | Translational Research Center
Molecular Biology Core Lab
This laboratory supports focused, small-scale PCR, low volume selective genotyping and haplotyping work; prepares investigator samples for high-throughput sequencing; DNA genotyping and RNA expression work.
  • PCR primer design (MacVector)
  • Mutation Analysis
     - DNA re-sequencing
     - Heteroduplex analysis
  • Restriction Fragment Analysis
  • Microsatellite Haplotying (Gene Mapper)
     - Loss of Heterozygosity
  • SNP genotyping by Real Time PCR (7900HT)
  • RNA
     - Total RNA isolation
     - Preparation of cDNA by Reverse-Transcription PCR
     - Gene expression analysis (Quantitative or Relative) by Real Time PCR (7900HT)


For more information, please contact the respective Technical Directors.
David Stokes, Ph.D., CHOP Technical Director
Phone: (215) 590-4752
Email: stokesdg@email.chop.edu
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University of Pennsylvania | School of Medicine