The Molecular Biology Core Lab

The Molecular Biology Core laboratory supports focused, small-scale PCR, low volume selective genotyping and haplotyping work, prepares investigator samples for high-throughput sequencing, and performs DNA genotyping and RNA expression analysis.

  • PCR primer design (MacVector)
  • Mutation Analysis
    • DNA re-sequencing
    • Heteroduplex analysis
  • Restriction Fragment Analysis
  • Microsatellite Haplotying (Gene Mapper)
    • Loss of Heterozygosity
  • SNP genotyping by Real Time PCR (ABI 7900HT)
  • RNA
    • Total RNA isolation
    • Preparation of cDNA by Reverse-Transcription PCR
    • Gene expression analysis (Quantitative or Relative) by Real Time PCR (ABI 7900HT)