The Molecular Biology Core Lab

The Molecular Biology Core laboratory supports focused, small-scale PCR, low volume selective genotyping and haplotyping work, prepares investigator samples for high-throughput sequencing, and performs DNA genotyping and RNA expression analysis.

• PCR primer design (MacVector)
• Mutation Analysis
  • DNA re-sequencing
  • Heteroduplex analysis
• Restriction Fragment Analysis
• Microsatellite Haplotying (Gene Mapper)
  • Loss of Heterozygosity
• SNP genotyping by Real Time PCR (ABI 7900HT)
• RNA
  • Total RNA isolation
  • Preparation of cDNA by Reverse-Transcription PCR
  • Gene expression analysis (Quantitative or Relative) by Real Time PCR (ABI 7900HT)